List of standard toxicological studies:
In vitro, ex vivo, in vivo eye corrosion/irritation studies
|
Isolated chicken eye test
method for identifying |
OECD Nr 438/ EU B.48 |
|
Short time exposure in vitro test method for identifying I) chemicals inducing serious eye damage II) chemicals not requiring classification for eye irritation or serious eye damage. |
OECD Nr 491/EU B.68 |
|
The
test method used within the study on the model of reconstructed human
cornea-like epithelium (RhCE), used for identifying chemicals not requiring
classification and labelling for eye irritation or serious eye damage. |
OECD Nr 492 |
|
Reconstructed human cornea-like epithelium
(RHCE) test method for eye hazard identification |
OECD Nr 492B |
|
Acute eye irritation/ eye corrosion test |
OECD Nr 405/EU B.5 |
In vitro, ex vivo, in vivo skin corrosion/irritation studies
|
In vitro skin corrosion: transcutaneous electrical
resistance test (TER). |
OECD Nr 430/ EU B.40 |
|
In vitro skin corrosion: reconstructed human epidermis
(RHE) test method |
OECD Nr 431/ EU B.40 |
|
In vitro skin irritation. The test on the model of
reconstructed human epidermis |
OECD Nr 439/ EU B.46 |
|
Acute skin irritation/ corrosion test. |
OECD Nr 404/ EU B.4 |
|
Biological evaluation of medical devices – Test for skin irritation in
vitro. |
ISO 10993-23 |
In vitro, in chemico, in vivo skin sensitisation studies
|
Skin sensitisation: local lymph node assay
(LLNA): BrdU-ELISA. |
OECD Nr 442B/EU B.51 |
|
In chemico skin sensitisation, direct peptide reactivity
assay (DPRA). |
OECD Nr 442C/ EU B.59 |
|
In vitro skin sensitisation: the test method with use
of ARE-Nrf2 Luciferase |
OECD Nr 442D/ EU B.60 |
|
In vitro skin sensitisation: Human cell line
activation test (H-CLAT). |
OECD Nr 442E/ EU B.71 |
|
Skin sensitisation study |
OECD Nr 406/ EU B.6 |
|
Delayed hypersensitivity assessment test using
the closed-patch method (Buehler Test). |
ISO 10993-10 |
In vitro cytotoxicity studies
|
In vitro cytotoxicity test. |
ISO 10993-5 |
In vitro phototoxicity
|
Phototoxicity - In vitro 3T3 NRU
phototoxicity test. |
OECD Nr 432 |
|
In vitro Phototoxicity - Reconstructed Human
Epidermis Phototoxicity test method |
OECD 498 |
In vitro, in vivo studies identifying endocrine-disrupting substances
|
Stably Transfected Human Estrogen Receptor-α
Transactivation Assay for Detection of Estrogenic Agonist and antagonist
Activity of Chemicals using the hERα-HeLa-9903 cell line. |
OECD Nr 455 |
|
Uterotrophic Bioassay in Rodents |
OECD Nr 440/ B.54 |
|
Hershberger
bioassay in rats. Short-term screening test of (anti-) androgenic properties |
OECD Nr 441 B.55 |
In vitro, in vivo acute toxicity
|
Use of the 3T3 neutral red uptake cytotoxicity
test to estimate starting doses for acute oral systemic toxicity tests. |
OECD Series on Testing and Assessment No. 129 (2010) (ENV/JM/MONO(2010)20) |
|
Acute oral toxicity test. |
OECD Nr 420/ EU B.1.BIS |
|
Acute oral/ intraperitoneal/ subcutaneous/ intravascular
toxicity test - acute toxic class method. |
OECD Nr 423/ EU B.1.TRIS |
|
Acute dermal toxicity test. |
OECD Nr 402/ EU B.3 |
|
Acute inhalation toxicity test. |
OECD Nr 403 |
|
Acute inhalation toxicity – acute toxic class
method. |
OECD Nr 436/ EU B.52 |
|
Maximum tolerated dose test (MTD). |
- |
|
Single
dose toxicity study in rodents. |
ISO 10993-11 |
In vivo subacute, subchronic and chronic studies
|
Dose range finding test (DRF |
- |
|
Repeated dose 28-day oral/ intraperitoneal/
subcutaneous toxicity study in rodents |
OECD Nr 407/ EU B.7 |
|
Repeated dose dermal toxicity: 21/ 28 day
study |
OECD Nr 410/ EU B.9 |
|
Repeated dose 90-day oral toxicity study in
rodents |
OECD Nr 408/ EU B.26 |
|
Subchronic dermal toxicity: 90-day study |
OECD Nr 411/ EU B.28 |
|
Chronic toxicity studies |
OECD Nr 452/ EU B.30 |
|
Combined chronic toxicity/ carcinogenicity
studies |
OECD Nr 453/ EU B.33 |
In vivo neurotoxicity studies
|
Neurotoxicity study in rodents. |
OECD Nr 424/ EU B.43 |
In vivo developmental and reproduction studies
|
Prenatal developmental toxicity study |
OECD Nr 414/ EU B.31 |
|
Reproduction/ developmental toxicity screening
test. |
OECD Nr 421 |
|
Combined repeated dose toxicity study with the
reproduction/ developmental toxicity screening test. |
OECD Nr 422 |
|
Extended one-generation reproductive toxicity
study in rats |
OECD Nr 443/ EU B.56 |
|
Two-generation reproduction toxicity study |
OECD Nr 416/ EU B.35 |
In vitro, in vivo genotoxicity studies
|
Bacterial reverse mutation test (AMES test,
microplate format) |
w oparciu o OECD Nr 471/ EU B.13/14 |
|
In vitro micronucleus test on the mammalian cell line |
OECD Nr 487/ EU B.49 |
|
In vitro mammalian cell gene mutation tests using the
HPRT genes |
OECD Nr 476/ EU B.17 |
|
In vivo mammalian erythrocyte micronucleus test |
OECD Nr 474/ EU B.12 |
|
In vivo mammalian cell gene mutation test using the
thymidine kinase gene |
OECD Nr 490/ EU B.67 |
|
In Vivo Mammalian Alkaline Comet Assay |
OECD Nr. 489 |
In vivo carcinogenicity studies
|
Carcinogenicity studies |
OECD Nr 451/ EU B.32 |
|
Combined chronic toxicity/ carcinogenicity
studies |
OECD Nr 453/ EU B.33 |
In vivo local tolerance studies
|
Local tolerance test |
EMA/CHMP/SWP/2145/2000, ISO 10993-10 |
Toxicokinetics/pharmacokinetics studies
|
Toxicokinetics/pharmacokinetics
study |
OECD Nr 417/ EU B.436/ ICH S3A |
Other services
| Toxicological studies conducted according to the procedures agreed with the customer |
| Haematological studies |
| Biochemical studies |
| Hormone identification |
| Tissue and organ collection |
| Preparing histopathology slides and their scanning |
On request, other tests can be developed and performed.
For any additional questions contact us at sekretariat.pszczyna@ipo.lukasiewicz.gov.pl or planowanie.pszczyna@ipo.lukasiewicz.gov.pl
Toxicology Research Group
Toxicological tests performed at the IPO can be characterized as follows:
- they allow a toxicological evaluation of chemical substances contained in pharmaceutical products, veterinary medicinal products, plant protection products, food and feed additives, industrial chemical substances, etc.,
- they make it possible to elaborate models which allow one to observe chemical substances in the body and to explain the mechanisms of various toxic effects caused by them,
- they serve as a basis for a routine evaluation of new chemical compounds,
- their results are used to register and license industrial chemical substances in compliance with specific regulations.
